Preparation and Characterization of Polyhydroxybutyrate Scaffolds to be Used in Tissue Engineering Applications
نویسندگان
چکیده
Polyhydroxyalkanoates (PHA) are good alternatives on account of biocompatible and biodegradable properties to produce materials as scaffolds for engineered tissues. Polyhydroxybutyrate (PHB) which is a member of polyhydroxyalkanoate family have been widely used as a biomaterial for in vitro and in vivo studies due to its unique properties such as improved flexibility and processability. In this study polyhydroxybutyrate scaffolds were prepared for tissue engineering applications. In order to improve cell attachment on the scaffolds they were modified. During the modification three different immunologically inactive compounds, polyetyhylene glycol (PEG), 2-hydroxyethyl methacrylate (HEMA) and ethylenediamine (EDA) were used in radio frequency glow discharge (RFGD) plasma polymerization system. Morphological evaluations were obtained by using scanning electron microscopy. Obtained results showed high and interconnected porosity. In vitro weight loss profiles of the scaffolds were investigated by using gravimetric method and found to be influenced by PHB concentration used in the preparation of scaffolds. Their biological promotion of activities including cell attachment, morphology and proliferation on L929 mouse fibroblast cells were examined and cytotoxicity tests were performed at the last part of the study.
منابع مشابه
Comparing Behavior of Chondrocyte Cells on Different Polyhydroxybutyrate Scaffold Structure for Cartilage Tissue Engineering
Introduction: As the ability to repair cartilage tissue in body is limited, finding a suitable method for cartilage regeneration has gained the attention of many scholars. For this purpose, scaffold structure and morphology, along with cell culture on it, can be a novel method to treat cartilage injuries, osteoarthritis. Methods: In this study, polyhydroxybutyrate (PHB) is selected as the scaf...
متن کاملSynthesis and characterization of fiber reinforced polymer scaffolds based on natural fibers and polymer for bone tissue engineering application
A wide range of materials and scaffolding fabrication methods for bone tissue engineering have beenexplored recently. Fiber reinforced polymers (FRP) system appears to be a suitable system. By the exclusiveuse of biocompatible or bio-absorbable polymers and fibers, novel generation of scaffolds for applicationsin tissue engineering can be prepared. Mulberry Silk as highlighted...
متن کاملPreparation and characterization of PCL polymeric scaffolds coated with chitosan/ bioactive glass/gelatin nanoparticles using the tips methodology for bone tissue engineering
Objective(s): The present study aimed to prepare polycaprolactone (PCL) scaffolds with high porosity and pore interconnectivity, in order to copy the microstructure of natural bones using the thermally induced phase separation (TIPS) technique. Materials and Methods: The scaffolds were coated with chitosan (CH), bioactive glass (BG), and gelatin nanoparticles (GEL NPs) and assessed using ...
متن کاملPreparation and Characterization of Aligned and Random Nanofibrous Nanocomposite Scaffolds of Poly (Vinyl Alcohol), Poly (e-Caprolactone) and Nanohydroxyapatite
Nanofibrous scaffolds produced by electrospinning have attracted much attention, recently. Aligned and random nanofibrous scaffolds of poly (vinyl alcohol) (PVA), poly (ε-caprolactone) (PCL) and nanohydroxyapatite (nHA) were fabricated by electrospinning method in this study. The composite nanofibrous scaffolds were subjected to detailed analysis. Morphological investigations revealed that the...
متن کاملPhysicochemical Characterization of nano-clinoptilolite/-TCP /gelatin Scaffold and its Application in Periodontics
Background and aim: Due to the composite structure of the jaw bone, gelatin and beta-calcium phosphate (b-TCP) biomaterials have been used repeatedly in bone tissue engineering. Despite the desirable properties of scaffolds made, their application has been limited due to their poor mechanical properties and high degradability. The aim of this study was to investigate the effect of clinoptilolit...
متن کامل